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sacX [2018-07-26 14:02:23]
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sacX [2018-07-26 14:02:23]

trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component of the PTS (low affinity) and control of SacY activity
locus
BSU38410
pI
9.35
mw
48.86 kDa
protein length
459 aa Sequence Blast
gene length
1377 bp Sequence Blast
function
sucrose uptake and phosphorylation, control of SacY activity
product
trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component (low affinity)
essential
no
ec
2.7.1.69
synonyms
ipa-14r, sacS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.19|Utilization of sucrose]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.4|Control of PRD-type regulators]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.1|Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]

    • Gene

    Coordinates
    3,942,234 → 3,943,613

    The protein

    Catalyzed reaction/ biological activity

  • Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

    • Protein family

  • [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] permease, sucrose permease (Scr) family [Pubmed|10627040]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|531F132F7F6A878F1E1D56977B9898A14272349A|sacX]-[gene|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|sacY]
    description
    [Pubmed|2116367]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1400159], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|1400159], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY]: antitermination, in [regulon|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY regulon]

    • regulation

  • induction by sucrose (at high concentration) [Pubmed|8535520]
    • view in new tab

    Biological materials

    Mutant

  • BKE38410 (Δ[gene|531F132F7F6A878F1E1D56977B9898A14272349A|sacX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCAATTAAAACCTCCTT, downstream forward: _UP4_TAACTGGATTTATTCGATTT
  • BKK38410 (Δ[gene|531F132F7F6A878F1E1D56977B9898A14272349A|sacX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCAATTAAAACCTCCTT, downstream forward: _UP4_TAACTGGATTTATTCGATTT

    • References

  • 10627040,2105292,2116367,1400159,8535520